Borrowing DNA

Genetic Engineering





http://ecx.images-amazon.com/images/I/31ZVTI4tpEL._SY450_.jpg To make these glow in the dark mice, KJ took the glow in the dark gene out of a firefly and inserted into the genes for each mouse. She used a restrictive enzyme to cut out the firefly's genome. The sticky end was matched up with a part of the mouse's DNA. This gave the phenotype glow in the dark to these three mice. 

KJ's first experiment was an attempt to make a glow in the dark mouse. The restrictive enzyme E matched up to cut out of the source organism's genome. This is the correct restrictive enzyme because it leaves at G-G site and cleaves 4 single-stranded bases on each end. A sticky end is a part of DNA that is cut in a certain place to match up another strand of DNA. To cut the bacterial plasmid I used enzyme E because it cleaves at GG, and leaves a 4 single stranded base on each end. After this was finished, the plasmid carried the glow in the dark gene. The experiment worked! However, the mouse faced extreme trauma, deathly cancer, arthritis, and other internal issues. My mouse portrayed a luminous glow as it withered away to a dramatic fatal death.